Sam: Dude, I'm not enabling your sick habit. You're like one of those lab rats that push the pleasure button instead of the food button until it dies.
Dean: What are you talking about, I eat.
I love all the quotes, but this is (one of) my favorite!(:♥

well i have few but probably my fav is
Sam: Dude, im not using this ID.
Dean: Why not?
Sam: Because it says Bikini Inspector on it.

or sam just got off the phone with bobby
Sam: Well, we are not dealing anti claus.
Dean: What did bobby say?
Sam: Ah, that we're morons

There are way too many quotes that are awesome

Dean: Eat me. No wait you actually might

and the whole scene in supernatural christmas
Dean says: are you telling me the easter bunnies Jewish

aah, I love a lot of quotes, but my favorites are:

what were you thinking shooting casper in the face, you freak?

Dean : All I see is the light at the end of the tunnel.
Sam : That's hellfire, Dean.

and the best :

I think I'm adorable !

Sam Winchester:
Dean, there's ten times as much lore about angels as there is about anything else we've ever hunted.

Dean Winchester:
You know what, there's a ton of lore on unicorns too. In fact, I hear that they ride on silver moonbeams, and that they shoot rainbows out of their ass!

Sam Winchester:
Wait, there's no such thing as unicorns?

there are toooooo many good ones to just choose.

there are ALOT to choose just one but i saw "Devil's Trap" like 2 houres ago and i liked xP:

Dean:where's our father,Meg?!
Meg:you didn't Ask very nice..
Dean:where's our father,Bitch?!
Meg:jezz,you kiss your mother with that mouth!!!

The episode where Sam and Dean have to go back to one of their old schools where Dean and Sam are talking and Dean says "This whistle make me a god." So funny. :D XD

There are too many there is no way I could pick just one.

Castiel- "This isn't funny Dean, the voice says I'm almost out of minutes!"

Still like the oldie - I hope you're apple pie is freaking worth it!

well I got many..like "I lost my shoe" , "If you fudging touch me again I'll fudging kill you" ,,jeeez so many..can't remember all x]

its a cross betweeen when Sams drunk and when hes high

drunk - Sam - ur bossy
Dean - what ?
Sam ur bossy and short -hahah
Dean - ur you drunk ?
Sam - ya so stupid

ow and - i lost my shoe - the face he makes omg - so adorable

Sam high
Dean - are you ok
Sam - no im not ok ......... im alesome
Dean - did they give you something
Sam - ow yeah .........they gave me eveything - it spatakululer haha

Sam - maybe ur going crazy
Dean - im not crazy
Sam - come on you have been at lest ....half crazy for a long time... since hell or before that even
Dean - ok
Sam - hay come here - cause your my brother and i still lovea - bOOP

In the episode with the ghostfacers;

Dean: "Well see, about a year ago I... no wait... no no no no... hehe, no. I'm not gonna talk about my *beep* problems in a *beep* reality show!"

Or Cas;
Castiel: I don't understand your definition of good news...

Or Sam:
Sam: What are you gonna do? Poke her with a stick?
Dean: *considering*
Sam: Dude, you're not gonna poke her with a stick!

And SO much more! =3

Dean: I think i'm adorable this
this is my Fav.